Objective:

To propose a method that combines hardware adaptive optics optical coherence tomography (AO-OCT) with computational aberration correction (CAC) to extend depth of focus in retinal imaging.

Key Findings:

• CAC enables cellular-level resolution across the full retinal thickness from a single focus setting.
• The technique allows simultaneous visualization of multiple retinal layers in a single volumetric dataset.
• In healthy eyes, CAC provides clear visualization of photoreceptors even at 1.5° from the fovea.
• In a patient with multiple sclerosis, CAC-enhanced imaging revealed significant ganglion cell loss and improved cone photoreceptor delineation.

Interpretation:

CAC enhances image sharpness across retinal layers without the need for multiple focus acquisitions.

Limitations:

• Good acquisition quality remains essential for effective CAC.
• Computational demands are a consideration, although defocus-only correction can be performed quickly.

Conclusion:

The hybrid approach of combining hardware AO-OCT with CAC may enhance the diagnosis and monitoring of retinal diseases.